RT-PCR seemed to be gold standard for detections of nCoV-2 during first wave taking reference as the SARS-CoV-2 strain genome. But the mutations occurring at a very fast pace during these months and surge...
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Cq value, a necessary basic for RT-PCR in COVID-19 Testing
To detect a real signal from biological samples, some cycles of DNA replication is required which is called as “cycle quantification” ( Cq). It generally has a maximum of 35 cycles and for Drosten...
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RT-PCR, a targeted diagnostic tool for SARS-COV-2 or not ?
Charite, a Germany’s most influential virologist in respect of COVID-19 employee of Christian Drosten said that they did not use purified particles for testing virus. MagNA pure 96 system (Roche,Penzberg,Germany) was use to extract...
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