<\/p>\n\n\n\n
To detect a real signal from biological samples, some cycles of DNA replication is required which is called as \u201ccycle quantification\u201d ( Cq). It generally has a maximum of 35 cycles and for Drosten PCR test Cq is 45.<\/p>\n\n\n\n
As per MIQE(Minimum Information for Publication of Quantitative real-time PCR Experiments) guidelines, Cq values higher than 40 are suspected, as they are of low efficiency and are not to be reported. The MIQE guidelines are developed by Stephen A. Bustin, professor for Molecular Medicine and a world renowned expert in q PCR.<\/p>\n\n\n\n
Bustin said that Cq value too high gives false positives by detecting interactions of primers and fluorescent probes. In RT-PCR to detect a RNA in sample, first the RNA has to be converted to cDNA (complementary DNA) which is widely recognized as inefficient by Jessica Schwaber of Centre for Commercialization of Regenerative Medicine in Toronto. There is also problem with the amount of DNA converted from RNA in the above process.<\/p>\n\n\n\n
These are the basic inadequacies in RT-PCR tests which were blinded out due to financial and political pressures on WHO, CDC,FDA that lead to the application of Fatal Therapy.<\/p>\n","protected":false},"excerpt":{"rendered":"
To detect a real signal from biological samples, some cycles of DNA replication is required which is called as \u201ccycle quantification\u201d ( Cq). It generally has a maximum of 35 cycles and for Drosten PCR test Cq is 45. As per MIQE(Minimum Information for Publication of Quantitative real-time PCR Experiments) guidelines, Cq values higher than […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"footnotes":""},"categories":[3],"tags":[63,61,62,60,66,68,54,65,64,67],"class_list":["post-70","post","type-post","status-publish","format-standard","hentry","category-covid-19-updates","tag-a-necessary-basic-for-rt-pcr-in-covid-19-testing","tag-cdc","tag-cq-value","tag-fda","tag-miqe","tag-professor","tag-rna","tag-rt-pcr-2","tag-rt-pcr-test","tag-stephen-a-bustin"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t